Diagnostic value of the saliva immunoglobulin free light chains concentrations measured in primary Sjцgren's syndrome

Proliferation and hyperactivation of B-lymphocytes in the salivary glands is a feature primary Sj ц gren's syndrome (pSS). Detection saliva proteins synthesized by may be important diagnosis this disease. Objective: to evaluate diagnostic value measuring concentration immunoglobulin free light chains (FLC) patients with pSS. Material methods. The cross-sectional study included 24 pSS over age 18 years. PSS was diagnosed according 2016 ACR/EULAR classification criteria. control group consisted 11 healthy volunteers. Blood-salivary histohematic barrier permeability ratio for albumin, FLC measured. Quantitative determination blood performed enzyme immunoassay. An immunohistochemical biopsies minor (MSG) carried out quantitative assessment CD3+, CD4+, CD8+, CD20+, CD21+, CD68+, CD138+ cells. Mann–Whitney U-test used compare traits. Identification thresholds using ROC analysis method. operating characteristic curve plotted, area under curve, indicators specificity, sensitivity, accuracy were calculated. Results discussion. obtained values corresponded low albumin individuals. median concentrations ê ë volunteers 1.08 [0.58; 1.91], 1.038 [0.55; 2.03] mg/l 0.36 [0.32; 0.54], 0.35 [0.21; 0.52] mg/l, respectively. statistically significantly higher than (p<0.01). amount correlated rate unstimulated flow: r s =-0.483 (p=0.02), =-0.491 (p=0.017), respectively.A relationship found between -chains specific number cells: =0.733 (p=0.025). Statistically significant correlations mononuclear cells MSG have not been established.Based on results analysis, determined. Concentrations - -type 0.56 0.68 correspond 0.84 (95% confidence interval, CI 0.69–0.98) 0.83 0.71–0.97), sensitivity 79.2% 59.5–90.8) 75% 55.1–88), specificity 81.8% 52.3–96.8) 90.9% 62.3–99.5), respectively.Salivary compared receiving glucocorticoids (GC). groups did differ way terms clinical laboratory parameters. daily dose GC 10 [5; 10] mg prednisolone equivalent. There no differences these groups. Conclusion. Salivary-fixed FLCs are most likely produced localized stroma glands. Determination can proposed as test considered surrogate marker benign B-cell proliferation MSG. Therapy medium doses does affect saliva.